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LTP-RANKL |
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￥4000 ￥2500， 請(qǐng)電郵sales@lifetein.com.cn直接訂購！ |
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小鼠 |
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每瓶25μg活性蛋白 |
| O35235 |
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大腸桿菌重組表達(dá) |
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分子篩凝膠過濾分離 |
| 是 |
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根據(jù)其誘導(dǎo)小鼠單核細(xì)胞進(jìn)行破骨細(xì)胞分化的生物學(xué)效果測(cè)定，EC50約為50ng/ml |
| 冷凍干燥蛋白粉末 |
| 使用包含0.1%以上的牛血清白蛋白的無菌PBS緩沖液進(jìn)行復(fù)溶后使用 |
| 凍干粉可于零下20攝氏度或更低溫度下保存12個(gè)月；復(fù)溶制劑可在4攝氏度下保存兩周；避免反復(fù)凍融 |
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| A. The size-exclusion chromatography of recombinant mouse RANKL and the SDS-PAGE analysis. B. The bone marrow derived monocytes were cultured in the presence of M-CSF(25 ng/ml) and RANKL (50 ng/ml) for 6 days. The cells were then fixed with 4% PFA and stained by TRAP solution. The image showed the formation of big multiple nucleus TRAP positive osteoclasts formed after M-CSF and RANKL stimulation.
A. 重組小鼠RANKL蛋白的分子篩洗脫圖譜與SDS-PAGE分析。B. 重組小鼠RANKL的活性分析。來自骨髓的單核細(xì)胞在添加本品(50 ng/ml)與M-CSF (25 ng/ml)的培養(yǎng)基中培養(yǎng)6天并隨后進(jìn)行PFA固定與TRAP染色成像。該圖顯示了使用本品刺激后巨型多細(xì)胞核形態(tài)的破骨細(xì)胞（TRAP+）的形成。
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| For research use only! |
