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Breaking News: The Nobel Prize in Physiology or Medicine 2019 was awarded jointly to William G. Kaelin Jr, Sir Peter J. Ratcliffe and Gregg L. Semenza. One of the key peptides was made by LifeTein: HIF1a-P564: DLDLEMLAPYIPMDDDFQLR.

Highlights:

References using synthetic peptides and antibodies from LifeTein. See search results page from the Google Scholar.

Tan, L., Yin, T., Xiang, H. et al. Aberrant cytoplasmic expression of UHRF1 restrains the MHC-I-mediated anti-tumor immune response. Nat Commun 15, 8569 (2024).

The polyclonal antibody recognizing human phosphorylated Ser-661(mouse Ser-656) in UHRF1 was generated by LifeTein, LLC.

Greene SE, Huang Y, Fischer K, Rosa BA, Martin J, Mitreva M, et al. (2024) A novel antigen biomarker for detection of high-level of Loa loa microfilaremia. PLoS Negl Trop Dis 18(9): e0012461. https://doi.org/10.1371/journal.pntd.0012461

Polyclonal rabbit antibodies to Ll-Bhp-1 were commercially produced using recombinant Ll-Bhp-1 and purified by protein A affinity chromatography (LifeTein, Somerset, NJ, USA).

Padula AM. Immunoreactivity of eastern small eyed snake (Cryptophis nigrescens) venom towards species-specific antibodies of five medically important venomous Australian elapids. Aust Vet J. 2024 Jul 15. doi: 10.1111/avj.13356. Epub ahead of print. PMID: 39009475.

LifeTein produced a series of snake venom antibodies. Venom species used for rabbit IgG production: Tiger, Brown, Black, Death adder, and Taipan. The snake venoms were used for immunization of rabbits to generate polyclonal antibodies.

Protein A purified rabbit IgG specific for each venom species (Table 1) were purchased from a commercial supplier (Lifetein, Somerset, NJ, USA).

Holocentromeres can consist of merely a few megabase-sized satellite arrays Nature CommunicationsNat Commun 14, 3502 (2023). https://doi.org/10.1038/s41467-023-38922-7

LifeTein designed and synthesized a series of peptides: CENH3, MIS12 and NDC80. The peptides were used for immunization of rabbits to generate polyclonal antibodies.

... The peptide synthesis, immunization, and antibody purification were performed by LifeTein...

Richard R.Rustandi， et al. Characterization of gH/gL/pUL128-131 pentameric complex, gH/gL/gO trimeric complex, gB and gM/gN glycoproteins in a human cytomegalovirus using automated capillary western blots; Vaccine; Available online 3 July 2021, https://doi.org/10.1016/j.vaccine.2021.06.033

LifeTein generated Anti-gM and anti-gN antibodies for Vaccine Analytical Research Development and Vaccine Process Development Merck & Co., Inc., Kenilworth, NJ, USA. The rabbit polyclonal anti-gM and anti-gN antibodies were elicited by vaccinating animals with synthesized peptide sequences of gM (1-13aa and 345-372aa) and gN (61-101aa). Animal immunization and sera collection were performed at Lifetein, LLC (Hillsborough, NJ, USA). The sera were affinity-purified by its corresponding peptides.

Jia Shen. et al. EGFR modulates microRNA maturation in response to hypoxia through phosphorylation of AGO2. Nature 497, 383–387 (16 May 2013), doi:10.1038/nature12080

LifeTein helped designed and synthesized a series of phosphorylated peptides. Then the peptides were used for phospho-specific antibody productions. The phospo-specific antibodies by LifeTein were confirmed to react with the epidermal growth factor receptor (EGFR). The Hung's lab showed that AGO2-Y393 phosphorylation mediates EGFR-enhanced cell survival and invasiveness under hypoxia. These findings suggest that modulation of miRNA biogenesis is important for stress response in tumour cells.

... The following peptides were chemically synthesized for antibody production in mice (Lifetein), Elisa verification (LifeteinConc.) and peptide competition assay in immunohistochemistry (IHC)... Supplementary information

George R. Tiller, Potential Roles of Peroxidases in Caenorhabditis Elegans Innate Immunity. 2014

Custom peptide antibodies were provided by LifeTein to study SKPO-1. The SKPO-1 peroxidase domain peptide was synthesized by LifeTein. The polyclonal antibodies were raised in rabbits and then affinity purified to accomplish high specifications and concentrations. The antibodies were successfully used to localize the SKPO-1 protein in hypodermis. It was found that the peroxidase SKPO-1 contributes to the host immune response during infection.

... With respect to having a pAb raised for IHC, I designed a synthetic SKPO-1 peroxidase domain peptide to be chemically synthesized and have a pAb raised in rabbits against said peptide by Lifetein?. This endeavor was successful and is covered in Chapter 3...

Hui-Lung Sun. et al. Stabilization of ERK-Phosphorylated METTL3 by USP5 Increases m6 A Methylation; Mol Cell; 2020 Nov 19;80(4):633-647.e7. doi: 10.1016/j.molcel.2020.10.026.

LifeTein helped synthesized a series of phosphorylated peptides. Then the peptides were used for phospho-specific p-METTL3 (S43) antibody productions. Sun et al. demonstrate that activation of the ERK pathway promotes m6A methylation by phosphorylation of METTL3 and WTAP.

Natural variant of collagen-like protein A in serotype M3 group A Streptococcus increases adherence and decreases invasive potential. Infect Immun. 2015 Jan 5. pii: IAI.02860-14.

LifeTein made the affinity-purified rabbit polyclonal anti-SclA antibody. The purified streptococcal collagen-like protein A (SclA) antibodies demonstrated that a mutation in Group A Streptococcus carrier strains increase adherence and decrease virulence.

ERK Activation Globally Downregulates miRNAs through Phosphorylating Exportin-5. Cancer Cell. 2016 Nov 14;30(5):723-736. doi: 10.1016/j.ccell.2016.10.001.

LifeTein designed and synthesized a series of phosphorylated peptides. ELISA and dot blot analysis were performed for the verification of antibody specificity.

... Antibodies to the Thr345, Ser416, and Ser497 phosphorylation sites of XPO5 were generated in collaboration with Lifetein LLC... Supplementary information

• Vaccine, Available online 3 July 2021, Characterization of gH/gL/pUL128-131 pentameric complex, gH/gL/gO trimeric complex, gB and gM/gN glycoproteins in a human cytomegalovirus using automated capillary western blots
• Nature Communications, Volume 11, Article number: 956 (2020), A molecular switch regulating transcriptional repression and activation of PPARγ
• Cell, 2019, 8(12), 1631, Post-ER Stress Biogenesis of Golgi Is Governed by Giantin
• Cell, 2019, A Cell-Penetrating Scorpion Toxin Enables Mode-Specific Modulation of TRPA1 and Pain
• Nature Communications, Volume 9, Article number: 4687 (2018), A structural mechanism for directing corepressor-selective inverse agonism of PPARγ
• Nature Communications, Article number: 1969 (2018) doi:10.1038/s41467-018-04390-7, Immunogene therapy with fusogenic nanoparticles modulates macrophage response to Staphylococcus aureus
• Nature Chemical Biology, Volume 14, pages 538–541 (2018), doi:10.1038/s41589-018-0054-z. Membrane association of monotopic phosphoglycosyl transferase underpins function
• Nature Communications, Volume 9, Article number: 607 (2018), Cellular imaging by targeted assembly of hot-spot SERS and photoacoustic nanoprobes using split-fluorescent protein scaffolds
• Nature, Available online on 17 January 2018, doi:10.1038/nature25442, Monitoring T cell–dendritic cell interactions in vivo by intercellular enzymatic labelling
• Cell, Available online 11 January 2018, LXR/ApoE Activation Restricts Innate Immune Suppression in Cancer
• Science Advances, Park et al., Sci. Adv. 2018; 4: e1601453 12 January 2018, Membrane insertion of—and membrane potential sensing by—semiconductor voltage nanosensors: Feasibility demonstration
• Nature Scientific Reports, Article number: 14157 (2017), Connexin 43 Hemichannels Regulate the Expression of Wound Healing-Associated Genes in Human Gingival Fibroblasts
• Nature Communications, 8, Article number: 1403 (2017), Identification of a peptide recognizing cerebrovascular changes in mouse models of Alzheimer’s disease
• Cell, Volume 166, Issue 5, p1257–1268.e12, 25 August 2016, HIV-1 Integrase Binds the Viral RNA Genome and Is Essential during Virion Morphogenesis
• Cell, Volume 167, Issue 1, p248–259.e12, 22 September 2016, Portable, On-Demand Biomolecular Manufacturing
• Nature Communications, Published 25 July 2016, 7, Article number: 12276 doi:10.1038/ncomms12276, Regulation of claudin/zonula occludens-1 complexes by hetero-claudin interactions
• Nature Communications, Published 01 February 2016, 7, Article number: 10470 doi:10.1038/ncomms10470, Export of malaria proteins requires co-translational processing of the PEXEL motif independent of phosphatidylinositol-3-phosphate binding
• Nature, 529, 172–177 (14 January 2016) doi:10.1038/nature16475, An ID2-dependent mechanism for VHL inactivation in cancer
• Cell, Volume 163, Issue 2, 8 October 2015, Pages 419–431, An Adaptor Hierarchy Regulates Proteolysis during a Bacterial Cell Cycle
• Nature Communications, 2015, Article number: 7443, doi:10.1038/ncomms8443, Pharmacological repression of PPARγ promotes osteogenesis
• Nature Scientific Reports, 2015, Article number: 9641, doi:10.1038/srep09641, Ryanodine receptors are targeted by anti-apoptotic Bcl-XL involving its BH4 domain and Lys87 from its BH3 domain
• Cell, Volume 159, Issue 4, 6 November 2014, Pages 844–856, DOI: 10.1016/j.cell.2014.10.032. A Noncanonical Frizzled2 Pathway Regulates Epithelial-Mesenchymal Transition and Metastasis
• Nature Communications, Accepted 02 Aug 2014, doi:10.1038/ncomms5904, An endocytosis pathway initiated through neuropilin-1 and regulated by nutrient availability
• Nature, Accepted 06 May 2014, doi:10.1038/nature13452, Carbonic anhydrases, EPF2 and a novel protease mediate CO2 control of stomatal development
• Nature Materials, Accepted 14 April 2014, doi:10.1038/nmat3982, Etchable plasmonic nanoparticle probes to image and quantify cellular internalization
• Nature Communications, 5, Article number: 3571 doi:10.1038/ncomms4571, An alternate binding site for PPARγ ligands
• Nature, 497, 383–387, 2013, doi:10.1038/nature12080, EGFR modulates microRNA maturation in response to hypoxia through phosphorylation of AGO2
• Nature Communications, doi:10.1038/ncomms3261, Aug, 2013, Disrupting malaria parasite AMA1–RON2 interaction with a small molecule prevents erythrocyte invasion
• Biochemistry, 2013, DOI: 10.1021/bi401034q, Mechanistic Basis for the Potent Anti-angiogenic Activity of Semaphorin 3F
• PNAS, 2012, doi:10.1073/pnas.1119073109, Structures of KIX domain of CBP in complex with two FOXO3a transactivation domains reveal promiscuity and plasticity in coactivator recruitment
• Cell, 2012, Volume 151, Issue 6, 1243-1255, Piwi-Interacting RNAs Protect DNA against Loss during Oxytricha Genome Rearrangement
• Nature Immunology, 2011, doi:10.1038/ni.2107, The carboxypeptidase ACE shapes the MHC class I peptide repertoire
• Journal of Virology, 2013, doi: 10.1128/JVI.00814-13, Cross-inhibition of ChikunGunya virus fusion and infection by alphavirus E1 domain III proteins

	Nature, Accepted 06 May 2014, doi:10.1038/nature13452, Carbonic anhydrases, EPF2 and a novel protease mediate CO2 control of stomatal development
	Nature Materials, Accepted 14 April 2014, doi:10.1038/nmat3982, Etchable plasmonic nanoparticle probes to image and quantify cellular internalization
	Nature Communications, 5, Article number: 3571 doi:10.1038/ncomms4571, An alternate binding site for PPARγ ligands
	Nature, 497, 383–387, 2013, doi:10.1038/nature12080, EGFR modulates microRNA maturation in response to hypoxia through phosphorylation of AGO2
	Nature Communications, doi:10.1038/ncomms3261, Aug, 2013, Disrupting malaria parasite AMA1–RON2 interaction with a small molecule prevents erythrocyte invasion
	Biochemistry, 2013, DOI: 10.1021/bi401034q, Mechanistic Basis for the Potent Anti-angiogenic Activity of Semaphorin 3F
	PNAS, 2012, doi:10.1073/pnas.1119073109, Structures of KIX domain of CBP in complex with two FOXO3a transactivation domains reveal promiscuity and plasticity in coactivator recruitment
	Cell, 2012, Volume 151, Issue 6, 1243-1255, Piwi-Interacting RNAs Protect DNA against Loss during Oxytricha Genome Rearrangement
	Nature Immunology, 2011, doi:10.1038/ni.2107, The carboxypeptidase ACE shapes the MHC class I peptide repertoire
	Journal of Virology, 2013, doi: 10.1128/JVI.00814-13, Cross-inhibition of ChikunGunya virus fusion and infection by alphavirus E1 domain III proteins

Srinivasan, P. et al. Disrupting malaria parasite AMA1–RON2 interaction with a small molecule prevents erythrocyte invasion. Nat. Commun. 4:2261 doi: 10.1038/ncomms3261 (2013).

Biotin-RON2, a synthetic biotinylated 42 mer peptide, was synthesized by LifeTein. Synthetic peptides are crucial in the process of understanding protein-protein interactions. Using a high throughput screen method, Prakash Srinivasan assessed the inhibitors that block interactions between AMA1-RON2. He shows that the inhibitor binds to AMA1 and prevents its interaction with RON2. Such inhibitors of merozoite invasion used in combination with existing antimalarials hold great promise as a novel therapeutic approach in the fight against malaria.

... Biotin-tagged RON2 peptide(DITQQAKDIGAGPVASCFTTRMSPPQQICLNSVVNTALSTSTQSAMK) with the two cysteines cyclized was synthesized and puri?ed (95% pure) by LifeTein, LLC USA (New Jersey, USA) ...

ET Smith, DA Johnson, Protein Science, 2013, Human enteropeptidase light chain: Bioengineering of recombinants and kinetic investigations of Structure and function, DOI: 10.1002/pro.2239

Synthetic peptides by LifeTein were used as substrate to study the protein structure and function. ET Smith and DA Johnson found that the positively charged peptides could contribute the substrate specificity in human enteropeptidase. It shows importance of the charge-charge interactions in the substrate binding pocket. For example, peptide DDDDK and DDDDR could have different specificities for substrates.

... The extended peptide substrates GD4K-pNA and GD4R-pNA (LifeTein) were used at concentrations between 0.02-1.0 mM and 5-100 μM, respectively. Reactions were initiated by adding enzyme to ...

Paul M. Arnaboldi, et al. An outer surface protein C (OspC) peptide derived from Borrelia burgdorferi sensu strico as a target for the serodiagnosis of early Lyme disease, Published ahead of print 30 January 2013, doi: 10.1128/CVI.00608-12

LifeTein advanced the research on serodiagnostic assays for Lyme disease. The linear epitopes OspC1 was synthesized by LifeTein. This highly conserved 20 amino acid peptide epitope detected specific IgM and/or IgG in 60 out of 98 serum samples. This OspC1 peptide could have value as a component of a multi-peptide Lyme disease serological assay with significantly improved capabilities for the diagnosis of early infection. OspC1 was proved to have higher specificity than the commercial available OspC peptide.

... A positive signal was required to be at least 4x the FSI of the negative assay control. Peptides chosen for further analysis were produced by Lifetein, Inc. (South Plainfield, NJ), and were a minimum of 90% purity. ...