Polyarginine peptides, which are very efficient CPPs have been used for siRNA delivery into cells.

Stearylated octaarginine (Stearyl-R8), are able to encapsulate and shuttle nucleic acids through cell membranes without the need to covalently attach the nucleic acid to the arginine-containing carrier peptide.

Case Study: EGFP-expressing hippocampal neurons

Efficiency: 16 nmol/l siRNA& → 50% reduction in EGFP activity

Reference: T?nges, L, Lingor, P, Egle, R, Dietz, GP, Fahr, A and B?hr, M (2006). Stearylated octaarginine and artificial virus-like particles for transfection of siRNA into primary rat neurons. RNA 12: 1431–1438.

詳情請查看細(xì)胞穿透肽(cell penetrating peptides, CPPs)